MiniSeq Reagent Kit

MiniSeq System Reagent kits have a ready-to-use cartridge with two-channel SBS chemistry for cost-efficient sequencing, even for low sample numbers.

Overview

MiniSeq Reagent Kits bring the power and reliability of proven Illumina sequencing by synthesis (SBS) technology to labs of all sizes at an affordable price without sacrificing data quality. These kits offer:

  • Robust base calling and optimal signal-to-noise ratio

  • Ready-to-use reagent cartridges that provide a simple workflow and reduce chance for error 

  • Intuitive labeling and RFID-encoded reagents

Configurations for various applications

The MiniSeq Reagent Kits offer multiple options for both sequencing output and read length configurations to support a wide range of applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing. In addition, innovations to the MiniSeq Rapid Reagent Kit enable faster run times for NGS workflows to improve lab efficiency and provide a quicker time to results, compared to MiniSeq standard reagents. MiniSeq Rapid reagents reduce sequencing run times to < 5 hours, enabling faster detection of respiratory viruses and pathogens, including SARS-CoV-2.

MiniSeq Reagent Kit 

No. of Reads 

Kit Size (cycles) 

Output (max.) 

2 × 150 Output 

2 × 75 Output 

1 × 75 Output 

MiniSeq High Output Kit 25 M  75, 150, 300 
7.5 Gb 
7.5 Gb 
3.75 Gb 
1.875 Gb 
MiniSeq Mid Output Kit 
8 M 
300 
2.4 Gb 
2.4 Gb 
1.2 Gb 
600 Mb 
MiniSeq Rapid Kit  20 M 
100 
2 Gb 
N/A 
N/A 
N/A 

Gb = gigabases, M = millions


Specifications


Documentation

Support documentation

A dedicated support section is not currently available for this product

Resources

MiniSeq Rapid Reagent Kit (100 cycles)

20044338

Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 100-cycle single read, rapid run on the MiniSeq System.

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MiniSeq High Output Reagent Kit (75-cycles)

FC-420-1001

Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 75-cycle High Output run on the MiniSeq System.

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MiniSeq High Output Reagent Kit (150-cycles)

FC-420-1002

Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 150-cycle High Output run on the MiniSeq System.

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MiniSeq High Output Reagent Kit (300-cycles)

FC-420-1003

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a High Output run (up to 25M reads). Includes: High Output Reagent Cartridge (300 cycles), Flow Cell, and Hybridization Buffer.

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MiniSeq Mid Output Kit (300-cycles)

FC-420-1004

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 8M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Flow Cell, and Hybridization Buffer.

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FAQs

The MiniSeq flow cell is a single-lane flow cell, which requires that samples prepared for sequencing be combined into a single library pool. The number of samples that can be combined in a single library pool depends on the library prep method. See the documentation for your library prep kit for more information.

The MiniSeq Rapid Reagent Kit has a run time of < 5 hours while the standard reagent kits have a run time of 13 hours. This is a reduction in run time of nearly 60% when using the MiniSeq Rapid Reagent Kit and can be important for applications that require a faster response time.

No, it only supports single reads of 20M for a rapid sequencing run. However, both the MiniSeq High Output Reagent Kit and MiniSeq Mid Output Kit support paired-end reads. 

Simply load user-prepared library pools directly to the flow cell and insert the flow cell into the MiniSeq System. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

The MiniSeq Reagent kit is used to sequence prepared libraries that can be used for a wide range of applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing.
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